BM0322

This is a selective enrichment broth for the isolation of Salmonella spp. from pharmaceutical, food, dairy and environmental samples. Malachite Green and Magnesium Chloride are included in the formulation as selective agents due to their ability to inhibit most enteric organisms whilst allowing Salmonella spp. to multiply freely. Gram +ve bacteria and most other enteric bacteria, are typically susceptible to or inhibited by Malachite Green, the high osmotic pressure and/or the low pH of the medium.

It should be noted that S.typhi and S.choleraesuis are sensitive to Malachite Green and may therefore be inhibited. This medium conforms to the requirements of the Harmonised USP/EP/JP.

Products

4-Pack Stratus – 600nm

The Stratus plate reader has new and improved capabilities to optimize flexibility for sample data collection in multiple standard plate formats to serve the wide-ranging needs of scientific researchers.

The Stratus 4-Pack includes four Stratus plate readers.

4-Pack Custom Stratus – 496nm

The Stratus plate reader has new and improved capabilities to optimize flexibility for sample data collection in multiple standard plate formats to serve the wide-ranging needs of scientific researchers.

The Stratus 4-Pack includes four Stratus plate readers.

Stratus – 600nm

Custom Stratus – 650nm

Custom Stratus – 496nm

Universal Adaptor

The Cerillo Stratus adapter is precision-manufactured out of a high-quality, durable material designed to stand up to rigorous use in the lab. It allows a Stratus be secured to a universal shaker plate via screws, and also features high-friction silicone strips and powerful neodymium magnets. The adapter features a plate-size extrusion allowing it to fit securely to small shakers designed for a microplate.

ePlex RP2 Control M451

The ePlex RP2 Control M451 is intended for use as an external positive and negative quality control to monitor the performance of in vitro laboratory nucleic acid testing procedures for the qualitative detection of pathogens on the ePlex® Respiratory Pathogen Panel 2 (RP2) performed on the ePlex System (GenMark Diagnostics, Inc.).

ePlex BCID-GN Negative

The ePlex BCID-GN Negative is intended for in vitro use as a qualitative external quality control to monitor the amplification, detection and identification steps of the ePlex® Blood Culture Identification Gram-Negative (BCID-GN) Panel performed on the ePlex System (GenMark Diagnostics, Inc.).

ePlex BCID-GN Control M326

The ePlex BCID-GN Control M326 is intended for in vitro use as a qualitative external quality control to monitor the amplification, detection and identification steps of the ePlex® Blood Culture Identification Gram-Negative (BCID-GN) Panel performed on the ePlex System (GenMark Diagnostics, Inc.).

ePlex BCID-GP Negative

The ePlex BCID-GP Negative is intended for in vitro use as a qualitative external quality control to monitor the amplification, detection and identification steps of the ePlex® Blood Culture Identification Gram Positive Panel performed on the ePlex System (GenMark Diagnostics, Inc).

ePlex BCID-GP Control M323 v1.1

The ePlex BCID-GP Control M323 v1.1 is intended for in vitro use as a qualitative external quality control to monitor the amplification, detection and identification steps of the ePlex® Blood Culture Identification Gram Positive Panel performed on the ePlex System (GenMark Diagnostics, Inc).

ePlex BCID-FP Negative

The ePlex BCID-FP Negative is intended for in vitro use as a qualitative external quality control to monitor the amplification, detection and identification steps of the ePlex® Blood Culture Identification Fungal Pathogen (BCID-FP) Panel performed on the ePlex System (GenMark Diagnostics, Inc).

ePlex BCID-FP Control M320 v1.1

The ePlex BCID-FP Control M320 v1.1 is intended for in vitro use as a qualitative external quality control to monitor the amplification, detection and identification steps of the ePlex® Blood Culture Identification Fungal Pathogen (BCID-FP) Panel performed on the ePlex System (GenMark Diagnostics, Inc).

Potassium Permanganate

Flourescence staining with Dagatronics Flourescence Staining Kit allows to detect mycobacteria, also called AFB(Acid-Fast-Bacilli) According to a WHO recommended technical.

Acid Alcohol for FR

Flourescence staining with Dagatronics Flourescence Staining Kit allows to detect mycobacteria, also called AFB(Acid-Fast-Bacilli) According to a WHO recommended technical.

Auramine

Flourescence staining with Dagatronics Flourescence Staining Kit allows to detect mycobacteria, also called AFB(Acid-Fast-Bacilli) According to a WHO recommended technical.

Methylene Blue

Ziehl-Neelsen(Kinyoun) staining with Dagatronics Ziehl-Neelsen(Kinyoun) Staining Kit allows to detect mycobacteria, also called AFB (Acid-Fast-Bacilli) according to a WHOrecommended technical.

Acid Alcohol for ZN

Ziehl-Neelsen(Kinyoun) staining with Dagatronics Ziehl-Neelsen(Kinyoun) Staining Kit allows to detect mycobacteria, also called AFB (Acid-Fast-Bacilli) according to a WHOrecommended technical.

Carbol Fuchsin

Ziehl-Neelsen(Kinyoun) staining with Dagatronics Ziehl-Neelsen(Kinyoun) Staining Kit allows to detect mycobacteria, also called AFB (Acid-Fast-Bacilli) according to a WHOrecommended technical.

Acetone Alcohol

Gram staining with Dagatronics Gram Staining Kit allows to detect Gram positive and Gram negative according to a WHO recommended technical.

GRAM Iodine

Gram staining with Dagatronics Gram Staining Kit allows to detect Gram positive and Gram negative according to a WHO recommended technical.

Crystal Violet

Gram staining with Dagatronics Gram Staining Kit allows to detect Gram positive and Gram negative according to a WHO recommended technical.

Ziehl-Neelsen Auto Stainer

This GRAM Auto Stainer provides fast, easy and stable staining process for staining clinical specimens according to GRAM staining method by automated system control. This new innovated stainer features advanced functions and performances over manual staining or automated dip-type stainers, include simplicity and convenience. It can be provide safety and comfortable environment of test lab.

Colorex Listeria (ISO)

Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria. This is one such medium and is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from clinical and food samples. The medium is made selective by the inclusion of Lithium chloride, Ceftazidime, Polymixin B, Nalidixic acid (to suppress other bacteria) and Amphotericin B (to suppress yeasts and fungi). With the combination of both the chromogenic substrate and phospholipase C enzyme reactions, it is possible to differentiate L.monocytogenes from other Listeria spp. Users should be aware that some strains of L.ivanovii are capable of producing an opaque halo, highlighting the need to confirm presumptively identified colonies.

Min.Mod.Glu.Medium Agar

Mineral Modified Glutamate Medium with 1.5% Agar
A modification of Mineral Modified Glutamate Medium where 15g/Litre of Agar has been added to create a plating medium suitable for the isolation of E.coli from food samples. Using this medium during the resuscitation stage allows for the recovery of E.coli from frozen, dried, heat-processed or low pH foods.

Braziers CCEY Agar

Brazier’s CCEY Agar with 1% Lysed Horse Blood – Blood, Cycloserine, Cefoxitin, 4% Egg Yolk Emulsion
Based on Fastidious Anaerobe Agar, Cholic Acid and p-Hydroxyphenylacetic Acid are added to enhance the isolation and differentiation of Clostridium difficile from clinical specimens. Cholic Acid promotes spore germination and p- Hydroxyphenylacetic Acid enhances production of p-cresol, a distinctive metabolite of Clostridium difficile. The medium is made selective by the inclusion of Cefoxitin and D-Cycloserine and Egg Yolk emulsion is added to differentiate Clostridium difficile from the Lecithinase producing clostridia. Lysed Blood is also added which optimises the colony fluorescence when cultures are examined under UV light.

Mueller Hinton w 2% NaCl(25ml)

Approved by the National Committee for Clinical Laboratory Standards (NCCLS) in USA this medium is approved for use in antimicrobial sensitivity testing by the disc diffusion method and is recommended particularly for use with the Bauer-Kirby Technique It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim and controlled to ensure correct zone sizes with Tetracyline and Aminoglycoside antibiotics. It can be considered as an alternative to Iso-Sensitest Agar. This particular formulation has an additional 2% Sodium Chloride added to the medium making it suitable for the detection of resistance to Methicillin in staphylococci and it is included in the recommendations of British Society for Antimicrobial Chemotherapy (BSAC) for this purpose. It is not however recommended for testing of organisms requiring a CO2 enriched environment due to the pH effect on the medium. If incubation in a CO2 enriched environment is essential control organisms should be included to confirm that results have not been altered.

Colorex mSuperCARBA

Colorex™ mSuperCARBA™ is a selective chromogenic medium that has been developed for the detection and isolation of carbapenemase producing Enterobacteriaceae (CPE). Nosocomial infections due to CPE are particularly difficult due to the limited treatment options. Therefore, this medium is designed to simplify the detection of CPE carriers and to allow for improved monitoring of high risk patient groups. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. All presumptive positive colonies should be confirmed for carbapenemase production.

Typical colour reactions are as follows:

Escherichia coli – Red/Pink colonies;

Klebsiella spp., Enterobacter spp., & Citrobacter spp. – Metallic blue colonies;

Other Gram –ve CPE – Colourless colonies;

Carbapenem sensitive bacterial species, Gram +ve bacterial species & yeasts – Inhibited.

Colorex C.difficile

Chromogenic medium for detection of Clostridium difficile.

Clostridium difficile (C.difficile) is the leading cause of nosocomial infectious diarrheoa in adults. These infections occur mostly in patients who have both medical care and antibiotic treatment and have become more frequent and more difficult to treat in the last years due to the emergence of highly toxigenic C.difficile strains.

Although PCR has become the leading C.difficile detection technique, culture is essential for strain typing and antimicrobial susceptibility testing. CHROMagarTM C.difficile is a new fluorogenic culture medium, extremely sensitive and selective, especially designed to simplify and speed up (24h) the culture of C.difficile.

Colorex StrepB Agar

About a quarter of pregnant women in the UK are estimated to carry Streptococcus agalactiae. As a result of this, babies become colonized with Streptococcus agalactiae (GBS) during labour and birth; the vast majority are unaffected by this colonization, however, a small percentage become infected with conditions such as eye infections, pneumonia, septicaemia or meningitis. Colorex™ StrepB Agar is a chromogenic media that presumptively identifies Streptococcus agalactiae (mauve/red colonies) after 18-24 hours incubation in aerobic conditions. Enterococci are differentiated by the formation of blue colonies; other organisms are inhibited or colourless.

NB: Some strains of Group A, C & G streptococci may also produce mauve colonies. Therefore, final identification may require additional testing.

Colorex MRSA (15ml)

Colorex™ MRSA is a chromogenic medium for the selective isolation of Methicillin-Resistant Staphylococcus aureus (MRSA). The medium can be used for the routine screening of clinical specimens for MRSA from a variety of sampling sites such as the nose, throat and groin. The medium incorporates a nutritious peptone base medium and a number of selective agents to inhibit most Gram-negative and Gram-positive bacteria as well as yeasts and moulds. The chromogenic detection of specific enzyme activity leads to the formation of pink/mauve colonies indicating MRSA (including low level resistant and hetero-resistant strains) following incubation at 37°C for 18-24 hours. Other organisms, if present are indicated by blue or colourless colonies. Any presumptive isolates must be confirmed using serological and/or biochemical techniques available to the laboratory. The use of this chromogenic medium does not diminish the requirement for conventional antimicrobial susceptibility tests for the confirmation of methicillin resistance.

Limitations:

1. S.aureus strains that possess a low MIC to the selective agent present in the medium but are mec A negative may form colonies on the medium.

2. Some MRSA strains may form typical colonies surrounded by a matte halo. The formation of the halo serves no diagnostic function.

3. Certain methicillin-resistant coagulase-negative staphylococci (CNS) may produce characteristic colonies. In some cases differentiation may be achieved by examination of the colour of these colonies, as they may be considerably darker in colour (bluish purple to a very dark pink/magenta).

4. Certain bacterial species other than staphylococci may produce colonies with a characteristic colour.

Colorex VRE

Vancomycin-resistant Enterococcus (VRE) infections are especially aggressive and have been associated with mortality rates approaching 60% to 70%. They are now the second-leading cause of nosocomial infections in the U.S., and their prevalence is increasing worldwide. Resistance to vancomycin has the potential to be transferred from bacteria to bacteria. Cross-resistance is mediated by plasmids and transposons, which may transfer the genes associated with resistance to other much more aggressive pathogens, such as staphylococci and streptococci. Three principal types of vancomycin resistance are found in Enterococcus spp.; VanA, VanB and VanC genotypes. VanA and VanB types account for most significant infections in clinical settings, involving E.faecium and E.faecalis. VanC resistance is a low-level intrinsic resistance found in other Enterococcus spp. The Colorex™ VRE media is another chromogenic media in the Colorex™ range, enabling presumptive identification of vancomycin resistant Enterococci by the formation of mauve/pink coloured colonies (for VanA and VanB genotypes) and blue coloured colonies (for VanC genotypes) after 18-24 hours incubation.

Colorex Staph aureus

This is a chromogenic medium for the isolation and presumptive identification of Staphylococcus aureus. Mauve colonies indicate Staph aureus following incubation (18 – 24 hours) at 37°C, other organisms, if not inhibited, are indicated by blue or colourless colonies. Studies have suggested that this media has a specificity and sensitivity of 99.4% and 95.5% respectively (Gaillot et al 2000).

Colorex O157 CT

Colorex 0157 with Cafixime & Tellurite

This medium replaces the conventional Sorbitol MacConkey Agar that is reputed for high levels of false positives and the difficulty of colonial interpretation and differentiation. Colorex O157 is a chromogenic medium with a very high specificity (98% according to K.A. Bettelheim, 1998 J.Appl.Microbiol.85:425-428) for E.coli O157. To reduce the level of background flora, the medium is made selective by the addition of Cefixime and Potassium tellurite. Positive colonies exhibit a mauve colouration enabling easy interpretation amongst blue or colourless colonies of other bacteria.

Colorex Candida

In recent years there has been an increase in the number of immuno-compromised patients, which has in turn led to an increased rate of infections associated with Candida species. There were 2151 reported cases of candidaemia in 2016 with C.albicans accounting for 42%, C.glabrata for 25%, C.parapsilosis for 9% and C.tropicalis for 3% of infections in England, Wales and Northern Ireland.(1)

COLOREX™ Candida was formulated specifically for the detection and isolation of clinically significant Candida spp. by means of colonial colour and morphology within 48hrs. COLOREX™ Candida allows for the recognition of a minor Candida population within a mixed population as well as the pre-dominant species thereby allowing for a patient specific treatment plan at the earliest possible opportunity. Most bacterial species will be inhibited due to the inclusion of chloramphenicol.

C.albicans – Green colonies

C.tropicalis – Metallic blue colonies

C.glabrata – Mauve to pink colonies

C.krusei – Large fuzzy pink colonies

Limitations

Definitive identification requires additional testing of isolates (e.g. MALDI-TOF). C.glabrata and C.parapsilosis cannot be readily distinguished on this particular medium. C.dubliniensis will form dark green colonies on COLOREX™ Candida so additional testing is required to confirm presence in the specimen. C.auris isolates will grow on this medium but the colony colour may vary from white to pale purple/pink so further testing will be required to confirm identification.

Primary UTI Opaque

Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed with the aim of simplifying the differentiation and presumptive identification of the main organisms usually found in Urinary Tract Infections. Based on the traditional CLED Medium, to prevent the swarming of Proteus spp, two chromogens are present in the medium. One allows the detection of enterococci giving rise to blue colonies whilst the second results in purple colonies of E. coli. Phenylalanine and Tryptophan are also included as indicators of Tryptophan deaminase activity producing brown colonies of Proteus spp. This media is an opaque version to aid differentiation and presumptive identification of the bacteria isolated.

Primary UTI

This is a chromogenic medium based on CLED that has been developed to allow differentiation and presumptive identification of organisms typically found in urinary tract infections. Reduced electrolyte concentration prevents swarming of Proteus spp. A sophisticated binary chromogenic system and supplementation with tryptophan allows differentiation of Enterococcus spp. (turquoise colonies), Proteus spp. (clear colonies with a brown halo), Enterobacter spp. (metallic blue colonies), Staphylococcus spp. (white colonies), and E. coli (purple colonies).

Colorex ESBL

Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the Enterobacteriaceae. Colorex™ ESBL (Extended Spectrum Beta-Lactamase) medium has been developed for the isolation of ESBL – producing organisms with the aim of simplifying the differentiation and presumptive identification of the causative organism. It should be noted that other non-ESBL and AmpC isolates will be inhibited on this medium reducing the incidence of false positives. The distinctive colonial colouration of the various species can reduce the need for additional follow up testing allowing many positive results to be issued within 24 hours of receipt of the sample. If necessary an Indole test for confirmation of Escherichia coli and TDA test for Proteus spp. can be performed directly from the medium.

Typical colour reactions are as follows:

Escherichia coli – Red colonies;

Proteus spp., Providencia spp. & Morganella spp. – Clear colonies with a brown halo;

Klebsiella spp., Enterobacter spp, Serratia spp. & Citrobacter spp. – Metallic blue colonies;

Salmonella spp. & Acinetobacter spp. – Clear colonies; Gram +ve bacterial species and yeasts – Inhibited.

ISA sensitivity test agar 25ml

A defined medium suitable for antimicrobial susceptibility testing this medium has been approved for use in the BSAC method. Most organisms will grow on this medium and the very low levels of Trimethoprim and Sulphonamide antagonists means that is not necessary to add Lysed Horse Blood when testing most organisms.

Sorbitol Mac & Cefix./Tell.

Sorbitol MacConkey with Cefixime & Tellurite (CT-Smac)
This is a selective differential medium for the isolation of Escherichia coli 0157:H7. It differs from other MacConkey medium in that Lactose has been replaced by Sorbitol. As Escherichia coli 0157:H7 does not ferment Sorbitol it produces pale translucent colonies whereas most other strains of Escherichia coli are Sorbitol positive and produce pink colonies. The medium is made more selective by the addition of the antimicrobial Cefixime and Potassium Tellurite.

R.P.M.I. Medium

RPMI Medium for E-Test
RPMI Medium is recommended for use in anti-fungal susceptibility testing of yeasts from clinical isolates using the E-Test method. The medium is based on a simple Glucose Agar with added RPMI-1640 Medium (without Sodium Bicarbonate & Phenol Red), which supplies the necessary vitamins and amino-acids, and MOPS (3-(Morpholino)propanesulfonic Acid) Buffer to maintain the medium pH during incubation.

Haemophilus Test Medium (25ml)

This is a medium specifically intended for sensitivity testing of Haemophilus influenzae and Haemophilus parainfluenzae. Based on Mueller Hinton Agar with added Yeast Extract the medium is enriched by the addition of Haemin and NAD (Nicotinamide Adenine Dinucleotide (V Factor)). It contains very low levels of antimicrobial antagonists that allow testing of Sulphonamides and Trimethoprim to be performed with confidence and is recommended by the Clinical and Laboratory Standards Institute (CLSI).

Blood Agar w Neomycin

Columbia Agar Base with 7% Horse Blood & 50mg/L Neomycin
Based on Columbia Agar enriched with 7% Horse Blood this formulation has been modified to include Neomycin, which will inhibit most gram-positive and gram-negative aerobes, making it suitable for use as a selective medium for the isolation of many anaerobes.

Campylobacter Sel. (Preston)

Campylobacter Selective Agar Preston Supplement
This is one of several selective media available for the isolation of Campylobacter spp in clinical, food and environmental laboratories. The medium is enriched with Lysed Horse Blood and made selective by the inclusion of Cefoperazone, to suppress other enteric organisms, and Amphotericin to suppress yeast & fungal growth.

Mueller-Hinton w 5% SB (25ml)

Mueller-Hinton Agar is a defined medium used primarily in Antimicrobial Sensitivity Testing using the disc diffusion technique described by Bauer-Kirby. It is approved as the definitive medium for this purpose by the National Committee for Clinical Laboratory Standards (NCCLS) in USA. It is low in Tetracycline antagonists and although the majority of organisms will grow on the medium without the need for additional supplementation this particular formulation has been enriched with Defibrinated Sheep Blood (5%) making it suitable for use with the more fastidious organisms including many of those that are dependant on Thymine and Thymidine for their growth. The medium can also be used for the isolation of Neisseria spp.

NB: In view of the possible effect on the pH of the medium when used with organisms requiring incubation in a CO2 enriched environment it is essential to include control organisms with the test plates.

BHI & VANCOMYCIN (25ml)

Brain Heart Infusion Agar with Vancomycin
This is a very nutritious general-purpose medium made selective by the inclusion of Vancomycin (6mgs/L) and is therefore suitable for the isolation of Vancomycin resistant organisms. Such as Streptococci and Staphylococci species.

Brain-Heart Infusion Agar

Brain Heart Infusion Agar
This is a very nutritious general-purpose medium suitable for the isolation of most organisms including many fastidious anaerobes. It is particularly recommended for streptococci and neisseria.

FAA with VANC & Nalidixic Acid

Fastidious Anaerobe Agar with 7% Horse Blood, Vancomycin (6mg/L) & Nalidixic Acid (10mg/L)
This is a selective medium for the isolation of gram-negative anaerobes from clinical specimens. The base medium, Fastidious Anaerobe Agar, is complex and includes detoxification agents (Starch & Sodium Bicarbonate), growth enhancing agents (Cysteine, Arginine, Vitamin K, Sodium Succinate, Glucose and pyrophosphate) as well as Haemin to encourage pigment production where appropriate. Sodium Pyruvate is also included to help neutralise Hydrogen Peroxide. The medium is made selective, by the inclusion of Naladixic Acid and Vancomycin and further enriched by the addition of 7% Horse Blood.

Baird Parker Agar

Baird Parker Agar with 5% Egg Yolk Tellurite
Baird Parker Agar is a selective medium for the isolation and presumptive identification of coagulase-positive staphylococci. This medium is used extensively for detecting Staphylococcus aureus in foods, dairy products, and other materials. The medium is made highly selective by the inclusion of Lithium Chloride and Potassium tellurite. The Potassium tellurite inhibits most coliforms and is reduced by staphylococci giving rise to black colonies. Glycine and Sodium Pyruvate are included as growth enhancers while the pyruvate also acts as a neutraliser of toxic peroxides.

NB: Any black colonies (with or without the halo) on this medium must be confirmed as Staphylococcus aureus by further tests (e.g. Coagulase Test or Latex Agglutination etc.)

Czapek Dox Agar

This is a defined medium primarily for the cultivation of fungi and bacteria that are capable of utilising Sodium Nitrate as their sole source of nitrogen. If made more acidic by adjusting to pH 3.5 it can also be used for isolation of acidophilic yeasts. The medium has also been used for the identification of Candida albicans by chlamydospore production.

TBX Agar

Tryptone Bile X (TBX) – Glucuronide Agar
Recent developments in culture media have given rise to the use of chromogenic substrates as a means of differentiating bacteria particularly among the coliform group of organisms. This is one such medium and has been developed as a selective medium for the isolation and enumeration of Escherichia coli in food samples. Based on Tryptone Bile Agar it incorporates the chromogenic agent X-glucuronide, which detects glucuronidase activity, the same enzyme as is detected by MUG reagent. Escherichia coli can be differentiated from other coliform organisms by the presence of glucuronidase resulting in the colonies being blue/green while the other coliforms are colourless. For information on the full technical detail as to the functionality of this medium reference should be made to the many publications available.

Columbia CNA w Horse Blood

This is a medium for the selective isolation of Staphylococcus spp. and Streptococcus spp. primarily from clinical specimens. Based on Columbia Agar Base enriched with 7% Horse Blood the medium is made selective by the inclusion of Colistin and Nalidixic Acid to suppress the growth of the majority of Gram negative bacteria.

Colorex Salmonella Plus

Colorex™ Salmonella Plus is a chromogenic media for the isolation and presumptive identification of Salmonella spp, S typhi, S.paratyphi and lactose positive Salmonella from foodstuffs. Any presumptive Salmonella spp. will produce a mauve colouration; other organisms will be blue or colourless in appearance. Cefsulodin is added as a selective agent to inhibit the growth of Pseudomonas and Aeromonas species. Any presumptive isolates must be confirmed using serological and biochemical techniques available to the laboratory. This product meets the requirements of ISO 6579:2002 standard.

Pseudomonas Sel. Agar C.F.C

Pseudomonas Agar Base with 1% Glycerol, Cephalothin, Fucidin & Cetrimide (CFC)
This is a selective medium for the isolation of Pseudomonas spp primarily in food, water and environmental samples. The medium uses Magnesium and Potassium salts to enhance pigment production and is made selective by the addition of CFC supplement. The presence of blue/green or brown pigmentation or fluorescence is indicative of presumptive Pseudomonas spp. It should be noted however that further testing must be carried out to confirm the full identity of the organism.

Potato Dextrose Agar (USP)

This medium is recommended for the detection and enumeration of yeasts and moulds in food and dairy products. It can also be used for the cultivation of fungi although with the prolonged incubation necessary cultures may become overgrown by bacteria. The low pH (5.6) suppresses the growth of most bacteria and the low mineral content ensures good pigment production by fungi where appropriate.

Plate Count Agar (Standard)

Plate Count Agar (APHA) (Standard Methods Agar, Tryptone Glucose Yeast Agar)
This medium is formulated to A.P.H.A. specification and intended for use in food, dairy and water bacteriology to perform Total Viable Counts. The agar is of high gel strength and is therefore suitable for use in pour plate techniques as well as surface inoculation.

Mueller Hinton w 5% DHB & NAD

Mueller-Hinton Agar is a defined medium used primarily in Antimicrobial Sensitivity Testing using the disc diffusion technique described by Bauer-Kirby. It has been approved as the definitive medium for this purpose by the European Committee on Anitmicrobial Susceptibility Testing (EUCAST). This medium contains low levels of thymidine and thymine and controlled levels of calcium and magnesium ions. Additional supplementation of the Mueller Hinton medium using 5% Horse Blood and 20mg/L of Nicotinamide adenine dinucleotide (NAD) makes it suitable for use with the more fastidious organisms such as Streptococcus pneumoniae and Haemophilus influenzae.

Mueller-Hinton (25ml)

Approved by the Clinical Laboratory Standards Institute (CLSI) in USA this medium can be considered as an alternative to Iso-Sensitest Agar for antimicrobial sensitivity testing by disc diffusion methods. It is low in Thymine and Thymidine and is therefore suitable for use in testing Sulphonamides & Trimethoprim without the addition of Lysed Blood.

Milk Agar Cetrimide (W)

This medium is used for the presumptive identification of Pseudomonas aeruginosa from water and environmental samples. Pseudomonas aeruginosa is presumptively identified by the characteristic green pigmentation of the colonies with hydrolysis of casein (clear zones around each colony).

Legionella G.V.P.C.

This is a selective medium for the isolation of Legionella spp is used primarily in water and environmental laboratories. The base agar contains Yeast Extract as a source of protein, Charcoal to neutralise growth-inhibiting substances and is supplemented with Ferric Pyrophosphate as a source of iron, L-cysteine hydrochloride and α-ketoglutarate to form amino acid and chelate respectively. ACES buffer is incorporated to maintain the optimal pH for growth. The medium is made selective by the addition of Glycine, Vancomycin & Polymyxin B to inhibit the majority of gram positive and gram negative organisms and Cycloheximide is also included to inhibit yeasts and fungi.

Kanamycin Aesculin Azide Agar

This is a selective medium for the isolation and enumeration of enterococci (Group D streptococci) primarily in food although it has found uses in other areas of bacteriology. The medium is made selective by the inclusion of Kanamycin and Sodium Azide while Aesculin and Ferric Ammonium Citrate act as the indicator system.

G.C. Sel & V.C.A.T.

There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of VCAT (Vancomycin, Colistin, Amphotericin B & Trimethoprim). Vancomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Amphotericin B to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.

G.C. Sel Agar with L.C.A.T.

There are a number of media available for the selective isolation of Neisseria gonorrhoeae of which this is one. Based on the medium developed by Thayer & Martin, it includes Corn Starch to absorb toxic metabolites and is buffered to maintain a neutral pH. The medium is made selective by the inclusion of LCAT (Lincomycin, Colistin, Amphotericin B & Trimethoprim). Lincomycin and Colistin inhibit the growth of the majority of contaminating organisms likely to be present. Trimethoprim is included to reduce the spreading of Proteus spp and Amphotericin B to inhibit yeasts and fungi. Yeast Extract and Glucose are also added as further enrichment along with laked horse blood to ensure an more luxuriant growth of Neisseria gonorrhoeae.

Mannitol Salt Agar (USP)

A selective medium for the isolation of Staphylococcus aureus. The high level of Sodium Chloride inhibits most other organisms and as most Staphylococcus aureus ferment Mannitol the inclusion of Phenol Red indicator gives rise to yellow colonies. This formulation complies with the requirements of the Harmonised USP/EP/JP. NB: Some strains of coagulase negative staphylococci can give rise to false positive results on this medium It is necessary therefore to confirm the identity of Staphylococcus aureus by other confirmatory tests (e.g. Coagulase test, Latex test etc.)

MacConkey Agar

This is a selective medium for the isolation and differentiation of bile tolerant gram-negative (enteric) and gram-positive (staphylococci and enterococci) organisms and has uses in all areas of bacteriology. It has the disadvantage that many strains of Proteus spp will spread on it and for this reason MacConkey Agar without Salt may be preferred.

Listeria Oxford Agar

One of several media available as a selective identification medium for the isolation and identification of Listeria monocytogenes in food & clinical laboratories. Using Columbia Agar as the base Lithium Chloride is included to inhibit enterococci and Acriflavine to inhibit some other gram positive and gram negative organisms that may be present in such specimens. It is made further selective by the addition of the antimicrobials Cefoxitin, Colistin & Fosfomycin with Amphotericin included to inhibit any yeasts present. Aesculin is present as an indicator since Listeria monocytogenes will hydrolyse it and the associated reaction with the Ferric Ammonium Citrate gives rise to a black precipitate around the colonies.

Hoyles Medium

A selective culture medium for the isolation and differentiation of Corynebacterium diphtheriae types, Hoyle’s medium allows for rapid growth of the organisms and normally 18 hours incubation should be sufficient for a diagnosis. As the medium is highly selective, inoculation should be by rubbing the swab (or other material) over the entire surface of the agar, there is no need to spread the inoculum with a loop indeed doing so can cause the organism to be missed especially when they are present only in small numbers.

C.L.E.D. Agar (Bevis)

CLED Agar (Bevis)
A modification by Bevis of the original CLED medium of Mackey & Sandys. This formulation uses a double indicator system (Andrade’s (Acid fuchsin) and Bromothymol blue) to improve differentiation of Lactose and Non-lactose fermenting organisms. The lack of Sodium Chloride also prevents the swarming of Proteus spp.

Bacitracin Chocolate Agar

Chocolate Agar with 7% Horse Blood & Bacitacin
A highly nutritious medium enriched with Horse Blood, where the blood has been ‘chocolated’ by heating the medium to 70°C. Suitable for the isolation of most pathogens including many fastidious organisms the addition of Bacitracin makes it is particularly suitable for the selective isolation of Haemophilus spp.

MacConkey Agar without Salt

Originally introduced for the isolation and differentiation of Lactose & Non-Lactose Fermenting enteric organisms the medium in this case has been modified to improve the isolation of staphylococci and enterococci. The absence of Sodium Chloride provides a low electrolyte medium that prevents spreading of most Proteus spp. Although recommended for use in the examination of urine samples in clinical laboratories it has uses in Food, Water and Dairy applications.

DNase Medium

DN’ase Medium
DNase Agar is used primarily in clinical laboratories to differentiate Staphylococcus aureus from other Staphylococci based on deoxyribonuclease activity. Following incubation of the plate and confirmation of a pure growth of Staphylococci, the surface of the medium is covered with a small quantity of 1M Hydrochloric Acid to precipitate the DNA. Staphylococcus aureus produce DNase enzymes that hydrolyse DNA resulting in a clear area around the colonies, described as being DNase positive, whereas coagulase negative Staphylococci do not produce clearing.

NB: As with most tests of this type a positive result should not be taken in isolation and other appropriate tests, e.g. Coagulase Test, Latex Agglutination etc, should be carried out.

D.C.A. (Hynes)

Desoxycholate Citrate Agar (DCA) (Hynes)
One of several media designed for the differential selective isolation of Salmonella and Shigella spp from clinical and environmental samples. Lactose is added to the medium together with Neutral Red indicator to assist in the differentiation of Lactose and Non-Lactose fermenting organisms. The medium is made selective by the inclusion of Sodium Desoxycholate and Sodium Thiosulphate, which will inhibit most gram-positive organisms. The Sodium Thiosulphate is also broken down by the enzyme thiosulphate reductase to form Sodium Sulphite and Hydrogen Sulphide. The Hydrogen Sulphide, if present, will in turn react with the ferric ions to produce a black precipitate of Ferrous Sulphide and give rise to the classical black centre of most Salmonellae. It has been suggested that this medium may be more suitable for secondary post-enrichment isolation while the original formulation is more appropriate for the primary inoculation of samples.

Yersinia Agar (CIN) VHP WRAP

This is a selective medium for the isolation and enumeration of Yersinia spp. in clinical and food samples. It is made selective by the inclusion of Sodium desoxycholate, Crystal violet and the antimicrobials Cefsulodin, Novobiocin and Irgasan. Mannitol is also included which Yersinia ferments giving a colony that produces a ‘Bull’s Eye’ appearance. The majority of other enteric organisms are inhibited but if they do grow they produce a large pinkish colony with an opaque halo.

X.L.D. Agar

Originally introduced as an aid to recovery of Shigella spp. XLD is also a first class medium for recovery of Salmonella spp. It differs from other media of this type in that it has less Sodium Desoxycholate as its selective agent. The indicator system is somewhat complex taking advantage of the fermentation or otherwise of three carbohydrates (Lactose Sucrose and Xylose) together with Lysine Decarboxylase and Sodium Thiosulphate as an indication of the presence or absence of Hydrogen Sulphide.